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Jpn. J. Appl. Phys. 42 (2003) pp. L798-L800  |Next Article|  |Table of Contents|
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Laser Irradiated Growth of Protein Crystal

Hiroaki Adachi^1,5,6, Kazufumi Takano^2,7, Youichiroh Hosokawa^3,5,6, Tsuyoshi Inoue^4,7, Yusuke Mori^1,5,6, Hiroyoshi Matsumura⁴, Masashi Yoshimura^1,5,6, Yasuo Tsunaka², Masaaki Morikawa², Shigenori Kanaya², Hiroshi Masuhara^3,5,6, Yasushi Kai⁴ and Takatomo Sasaki^1,5,6

¹Department of Electrical Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
²Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
³Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
⁴Department of Materials Chemistry, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
⁵Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
⁶CREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
⁷PREST, JST, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

(Received May 14, 2003; accepted for publication June 2, 2003)

We succeeded in the first ever generation of protein crystals by laser irradiation. We call this process Laser Irradiated Growth Technique (LIGHT). Effective crystallization was confirmed by applying an intense femtosecond laser. The crystallization period was dramatically shortened by LIGHT. In addition, protein crystals were obtained by LIGHT from normally uncrystallized conditions. These results indicate that intense femtosecond laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. The nuclei formation is possible primarily due to nonlinear nucleation processes of an intense femtosecond laser with a peak intensity of over a gigawatt (GW).

URL: http://jjap.jsap.jp/link?JJAP/42/L798/
DOI: 10.1143/JJAP.42.L798

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