Jpn. J. Appl. Phys. 45 (2006) pp. 4259-4264  |Previous Article| |Next Article|  |Table of Contents|
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High-Density and Highly Surface Selective Adsorption of Protein–Nanoparticle Complexes by Controlling Electrostatic Interaction

Kiyohito Yamada¹, Shigeo Yoshii¹, Shinya Kumagai¹, Isamu Fujiwara², Kazuaki Nishio¹, Mitsuhiro Okuda¹, Nozomu Matsukawa¹ and Ichiro Yamashita^1,2,3

(Received November 17, 2005; accepted February 5, 2006; published online May 9, 2006)

High-density cage-shaped proteins with inorganic cores were selectively adsorbed as a monolayer onto a 3-aminopropyl-triethoxysilane (APTES) layer on a Si substrate. The electrostatic interaction between the protein and substrate surface was studied and it was proven that protein adsorption density depends on the quantitative balance of surface charge on the substrate and protein. The combination of a highly positive APTES layer and moderately negative ferritin, Fer-4, achieved an adsorption density of 7.6×10¹¹ cm^-2 and the combination of the APTES layer and Listeria ferritin (Lis-fer) reached an adsorption density of 1.3×10¹² cm^-2. The adsorption process including the reduced charge of Lis-fer due to denaturation further enhanced the adsorption density up to 1.5×10¹² cm^-2, whereas no Lis-fer was adsorbed onto the SiO₂ surface under the same conditions. This new technique makes it possible to produce a nanodot monolayer with a density higher than 1×10¹² cm^-2, which can be applied to floating nanodot gate memories.

URL: http://jjap.jsap.jp/link?JJAP/45/4259/
DOI: 10.1143/JJAP.45.4259

References | Citing Articles (23)

1. I. Yamashita: Thin Solid Films 393 (2001) 12[CrossRef].
2. T. Hikono, Y. Uraoka, T. Fuyuki and I. Yamashita: Jpn. J. Appl. Phys. 42 (2003) L398[JSAP].
3. G. Yamazaki, Y. Uraoka, T. Fuyuki and I. Yamashita: J. Photopolym. Sci. Technol. 16 (2003) 439.
4. T. Kubota, T. Baba, S. Samukawa, H. Kawashima, Y. Uraoka, T. Fuyuki and I. Yamashita: Appl. Phys. Lett. 84 (2004) 1555[AIP Scitation].
5. S. Tiwari, F. Rana, H. Hanafi, A. Hartstein, E. F. Crabbé and K. Chan: Appl. Phys. Lett. 68 (1996) 1377[AIP Scitation].
6. S. Yoshii, K. Yamada, N. Matsukawa and I. Yamashita: Jpn. J. Appl. Phys. 44 (2005) 1518[JSAP].
7. T. Yoshinobu, J. Suzuki, H. Kurooka, W. C. Moon and H. Iwasaki: Electrochim. Acta 48 (2003) 3131.
8. H. Masuda, H. Hogi, K. Nishio and F. Matsumoto: Chem. Lett. 33 (2004) 812[CrossRef].
9. D. J. Price and J. G. Joshi: J. Biol. Chem. 258 (1983) 10873.
10. F. C. Meldrum, V. J. Wade, D. L. Nimmo, B. R. Heywood and S. Mann: Nature 349 (1991) 684[CrossRef].
11. F. C. Meldrum, T. Douglas, S. Lei, P. Arosio and S. Mann: J. Inorg. Biochem. 58 (1995) 59.
12. T. Douglas, D. P. E. Dickson, S. Betteridge, J. Charnock, C. D. Garner and S. Mann: Science 269 (1995) 54[Science].
13. K. K. W. Wong and S. Mann: Adv. Mater. 8 (1996) 928[CrossRef].
14. T. Douglas and V. T. Stark: Inorg. Chem. 39 (2000) 1828.
15. M. Okuda, K. Iwahori, I. Yamashita and H. Yoshimura: Biotechnol. Bioeng. 84 (2003) 187.
16. I. Yamashita, J. Hayashi and M. Hara: Chem. Lett. 33 (2004) 1158[CrossRef].
17. K. Iwahori, K. Yoshizawa, M. Muraoka and I. Yamashita: Inorg. Chem. 44 (2005) 6393.
18. R. Tsukamoto, K. Iwahori, M. Muraoka and I. Yamashita: Bull. Chem. Soc. Jpn. 78 (2005) 2075[CrossRef].
19. H. Sugimura, A. Hozumi, T. Kameyama and O. Takai: Surf. Interface Anal. 34 (2002) 550.
20. K. Iwahori and I. Yamashita: private communication.
21. M. Bozzi, G. Mignogna, S. Stefanini, D. Barra, C. Longhi, P. Valenti and E. Chiancone: J. Biol. Chem. 272 (1997) 3259.
22. M. Allen, D. Willits, M. Young and T. Douglas: Inorg. Chem. 42 (2003) 6300.
23. S. Kumagai, S. Yoshii, K. Yamada, I. Fujiwara, N. Matsukawa and I. Yamashita: J. Photopolym. Sci. Technol. 18 (2005) 495.