Jpn. J. Appl. Phys. 47 (2008) pp. 2270-2274  |Previous Article| |Next Article|  |Table of Contents|
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Combined Microfluidic–Microelectric Trap of Nanopolymeric Beading Conjugant in Continuous Flow

Ming-Wen Wang

(Received July 9, 2007; accepted January 9, 2008; published online April 18, 2008)

The selective sorting of biomolecules in biofluid has been carried out via coated beads selectively attached to the target molecules by an antigen–antibody reaction. Eventually, the conjugant must be trapped and separated from the mixture by applying other technologies. To obtain the nanopolymeric beading conjugant that can be trapped and separated from the biological mixture, a microfluidic component of a microchannel with electrodes has been designed and fabricated using a micro-electromechanical system technology. A local dielectrophoretic force obtained from nonuniform electric fields was used for manipulating and trapping a nanopolymeric beading conjugant in a continuous flow. The experimental studies show that the beads undergo positive dielectrophoresis when suspended in a continuous flow (Re=5.0) and exposed to AC fields at 100 kHz frequency. Using this device, the microelectrode provides a local dielectrophrosis field strong enough to manipulate and attract a nearby nanopolymeric beading conjugant in a continuous flow.

URL: http://jjap.jsap.jp/link?JJAP/47/2270/
DOI: 10.1143/JJAP.47.2270

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