Jpn. J. Appl. Phys. 47 (2008) pp. 2300-2305  |Previous Article| |Next Article|  |Table of Contents|
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Surface Modification of Gel-Free Microchannel Surface Electrophoresis Device for DNA Identification

Hyun Ho Lee and Yue Kuo¹

(Received April 20, 2007; accepted January 5, 2008; published online April 18, 2008)

A gel-free microchannel electrophoresis device for DNA separation and identification was studied. DNA fragments ranging from 3.5 to 21.2 kbp were effectively separated and identified. The channel's bottom surface was composed of silicon wafer or glass, and the channel wall was composed of SU-8 photoresist. The channel surface was modified with various solutions or plasmas. The separated DNA on the detection electrode was confirmed by electron spectroscopy for chemical analysis. DNA mobility on the glass substrate was higher than that on the Si substrate. In addition, the increase of the ionic strength of the solution on the device decreased the mobility of DNA. In summary, this new device is applicable to large DNA samples.

URL: http://jjap.jsap.jp/link?JJAP/47/2300/
DOI: 10.1143/JJAP.47.2300
KEYWORDS:microchannel device, DNA electrophoresis, SU-8, ESCA

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