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Jpn. J. Appl. Phys. 49 (2010) 105103 (5 pages)  |Previous Article| |Next Article|  |Table of Contents|
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Ultrasensitive Detection of Cymbidium Mosaic Potexvirus Using a Single-Wall Carbon Nanotube-Functionalized Quartz Crystal Microbalance

Yu-Shiun Chen1,5, Yao-Ching Hung2, Jin-Chern Chiou3, Hui-Liang Wang4, Hung-Shu Huang1, Li-Chia Huang2, and Guewha Steven Huang1

1Institute of Nanotechnology, Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, EE137, Hsinchu 300, Taiwan, Republic of China
2Departments of Obstetrics and Gynecology, School of Medicine, China Medical University and Hospital, 91 Hsueh-Shih Road, Taichung 404, Taiwan, Republic of China
3Institute of Electrical Control Engineering, National Chiao Tung University, 1001 University Road, EE772, Hsinchu 300, Taiwan, Republic of China
4Department of Biotechnology, National Kaohsiung Normal University, 62 Shen Jhong Road, Kaohsiung 824, Taiwan, Republic of China
5Institute of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, EE137, Hsinchu 300, Taiwan, Republic of China

(Received March 18, 2010; revised July 21, 2010; accepted August 8, 2010; published online October 20, 2010)

We have developed an ultrasensitive, convenient, real-time platform for detecting Cymbidium mosaic potexvirus (CymMV) based on single-wall carbon nanotube (SWNT)-functionalized quartz crystal microbalance (QCM) sensors. Functionalization was achieved by coating the QCM electrode with SWNTs, followed by 1,1'-carbonyldiimidazole-activated Tween 20 (CDI–Tween 20) modification and conjugation of antibodies. Sensitivity was enhanced from 2.18 to 11.5 Hz ng-1 when 0.1 µg mL-1 CymMV was applied. The low limit of detection of SWNT-functionalized QCM sensors was improved from 2.08 to 0.502 ng. The SWNT-functionalized QCM sensor was successfully used to quantify the amount of CymMV contained in infected orchid leaves. Compared to enzyme-linked immunosorbent assay (ELISA), SWNT-functionalized QCM sensors are fast, economical, and ultra-sensitive, with comparable sensitivities. The current study demonstrates the application of QCM sensors as a convenient platform to detect and quantify CymMV.

URL: http://jjap.jsap.jp/link?JJAP/49/105103/
DOI: 10.1143/JJAP.49.105103


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