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Jpn. J. Appl. Phys. 51 (2012) 04DL02 (6 pages)  |Previous Article| |Next Article|  |Table of Contents|
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Sub-micro-liter Electrochemical Single-Nucleotide-Polymorphism Detector for Lab-on-a-Chip System

Hiroyuki Tanaka1,2, Paolo Fiorini2, Sara Peeters2, Bivragh Majeed2, Tom Sterken2, Maaike Op de Beeck2, Miho Hayashi3, Hidenobu Yaku1,4, and Ichiro Yamashita1

1Advanced Technology Research Laboratory, Panasonic Corporation, Seika, Kyoto 619-0237, Japan
2IMEC vwz., Kapeldreef 75, B-3001 Leuven, Belgium
3R&D Center, Panasonic Healthcare Corporation, Toon, Ehime 791-0395, Japan
4Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe 650-0047, Japan

(Received September 25, 2011; accepted December 7, 2011; published online April 20, 2012)

A sub-micro-liter single-nucleotide-polymorphism (SNP) detector for lab-on-a-chip applications is developed. This detector enables a fast, sensitive, and selective SNP detection directly from human blood. The detector is fabricated on a Si substrate by a standard complementary metal oxide semiconductor/micro electro mechanical systems (CMOS/MEMS) process and Polydimethylsiloxane (PDMS) molding. Stable and reproducible measurements are obtained by implementing an on-chip Ag/AgCl electrode and encapsulating the detector. The detector senses the presence of SNPs by measuring the concentration of pyrophosphoric acid generated during selective DNA amplification. A 0.5-µL-volume detector enabled the successful performance of the typing of a SNP within the ABO gene using human blood. The measured sensitivity is 566 pA/µM.

URL: http://jjap.jsap.jp/link?JJAP/51/04DL02/
DOI: 10.1143/JJAP.51.04DL02


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