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Jpn. J. Appl. Phys. 51 (2012) 06FK01 (7 pages)  |Previous Article| |Next Article|  |Table of Contents|
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Microchip-Based Organophosphorus Detection Using Bienzyme Bioelectrocatalysis

Yong Duk Han, Chi Yong Jeong, Jun Hee Lee, Dae-Sik Lee1, and Hyun C. Yoon

Department of Molecular Science and Technology, Ajou University, Suwon, Gyeonggi 443-749, Republic of Korea
1BT Convergence Research Laboratory, ETRI, Daejeon 305-700, Republic of Korea

(Received November 7, 2011; accepted February 3, 2012; published online June 20, 2012)

We have developed a microsystem for the detection of organophosphorus (OP) compounds using acetylcholine esterase (AchE) and choline oxidase (ChOx) bienzyme bioelectrocatalysis. Because AchE is irreversibly inhibited by OP pesticides, the change in AchE activity with OP treatment can be traced to determine OP concentration. Polymer-associated ChOx immobilization on the working electrode surface and magnetic microparticle (MP)-assisted AchE deposition methods were employed to create an AchE–ChOx bienzyme-modified biosensing system. ChOx was immobilized on the micropatterned electrodes using poly(L-lysine), glutaraldehyde, and amine-rich interfacial surface. AchE was immobilized on the MP surface via Schiff's base formation, and the enzyme-modified MPs were deposited on the working electrode using a magnet under the microfluidic channel. The bioelectrocatalytic reaction between AchE–ChOx bienzyme cascade and the ferrocenyl electron shuttle was successfully used to detect OP with the developed microchip. This provides a self-contained and relatively easy method for OP detection. It requires minimal time and a small sample size, and has potential analytic applications in pesticides and chemical warfare agents.

URL: http://jjap.jsap.jp/link?JJAP/51/06FK01/
DOI: 10.1143/JJAP.51.06FK01


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